{"id":185,"date":"2018-07-19T22:48:04","date_gmt":"2018-07-19T19:48:04","guid":{"rendered":"http:\/\/2019.brs.org.tr\/?page_id=185"},"modified":"2018-07-21T21:30:47","modified_gmt":"2018-07-21T18:30:47","slug":"western-blot","status":"publish","type":"page","link":"https:\/\/2021.brs.org.tr\/index.php\/western-blot\/","title":{"rendered":"Western Blot"},"content":{"rendered":"

\"\"<\/p>\n

Proteinler yap\u0131sal ve h\u00fccresel metabolik s\u00fcre\u00e7lerin s\u00fcrd\u00fcr\u00fclmesinde fonksiyonel biyomolek\u00fcllerdir. Dizileri DNA da belirlenmi\u015f olan biyolojik olaylarda aktif olarak rol alan y\u00fcz binden fazla proteinoldu\u011fu varsay\u0131lmaktad\u0131r. Western Blot doku veya h\u00fccre k\u00fclt\u00fcr\u00fc gibi protein kaynaklar\u0131ndan elde edilen kar\u0131\u015f\u0131mdan hedeflenen \u00f6zel bir proteini ara\u015ft\u0131rma y\u00f6ntemidir. Bu y\u00f6ntemle numunelerdeki ara\u015ft\u0131r\u0131lmas\u0131 hedeflenen proteinin b\u00fcy\u00fckl\u00fc\u011f\u00fc, konsantrasyonu ve ara\u015ft\u0131rma gruplar\u0131na g\u00f6re nicelik k\u0131yaslamas\u0131 yap\u0131labilir.<\/p>\n

Western Blot tekni\u011fi yedi a\u015famada yap\u0131labilir;<\/p>\n

1. \u00d6rneklerin haz\u0131rlanmas\u0131: H\u00fccre veya dokulardan proteinlerin izole edilmesi.<\/p>\n

2. Y\u00fcr\u00fctme: \u0130zole proteinleri agaroz jelde elektrik alan etkisiyle y\u00fcr\u00fctme.<\/p>\n

3. Transfer: Agaroz jelde fazlara ayr\u0131lan proteinleri membrana aktarma.<\/p>\n

4. Bloklama: Mebrandaki non-spesifik proteinleri yakalama<\/p>\n

5. Antikor ile ink\u00fcbasyon: spesifik antikorlar kullanarak istenen proteinin yakalanmas\u0131.<\/p>\n

6. Substrat ile i\u015faretleme: Antikorun hedef proteine ba\u011fland\u0131\u011f\u0131 yeri g\u00f6steren bir bant (\u015ferit) meydana getirmesi.<\/p>\n

7. G\u00f6r\u00fcnt\u00fcleme: Enzim ba\u011fl\u0131 antikorun substrat\u0131 ile i\u015faretlemesiyle olu\u015fan bantlardan \u0131\u015f\u0131k yay\u0131l\u0131r.<\/p>\n

8. Yay\u0131lan \u0131\u015f\u0131k ya R\u00f6ntgen (X-Ray) filmine aksettirilirerek veya bir CCD kamera ile orataya \u00e7\u0131kan protein bantlar\u0131 g\u00f6r\u00fcnt\u00fclenir.<\/p>\n

<\/h4>\n

Western Blot\u00a0Kurs Program\u0131<\/strong><\/h3>\n

1. Ders Western Blot Tekni\u011fi Hakk\u0131nda Genel Bilgi<\/p>\n

\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0130lkeler<\/p>\n

\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Veri Analizleri<\/p>\n

\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Sorun Giderme<\/p>\n

2. Ders Western Blot Ekipmanlar\u0131n\u0131n tan\u0131t\u0131m\u0131
\n3. Ders \u00d6rnek ve Jel haz\u0131rlanmas\u0131
\n4. Ders \u00d6rnek Y\u00fcklemeleri ve Elektroforez i\u015flemi
\n5. Ders Elektro-Transfer i\u015flemi(Proteinlerin membrana g\u00f6\u00e7\u00fc)
\n6. Ders Membran Bloklama ve Primer Atibody ile ink\u00fcbasyon
\n7. Ders Sekonder Antibody ile ink\u00fcbasyon,
\n8. Ders G\u00f6r\u00fcnt\u00fcleme (Karanl\u0131k oda uygulamalar\u0131 ve G\u00f6r\u00fcnt\u00fcleme cihaz\u0131 uyg.)
\n9. Ders Sonu\u00e7 yorumlamalar\u0131
\n10. Ders Tart\u0131\u015fma ve Tavsiyeler<\/p>\n","protected":false},"excerpt":{"rendered":"

Proteinler yap\u0131sal ve h\u00fccresel metabolik s\u00fcre\u00e7lerin s\u00fcrd\u00fcr\u00fclmesinde fonksiyonel biyomolek\u00fcllerdir. Dizileri DNA da belirlenmi\u015f olan biyolojik olaylarda aktif olarak rol alan y\u00fcz binden fazla proteinoldu\u011fu varsay\u0131lmaktad\u0131r. Western Blot doku veya h\u00fccre k\u00fclt\u00fcr\u00fc gibi protein kaynaklar\u0131ndan elde edilen kar\u0131\u015f\u0131mdan hedeflenen \u00f6zel bir proteini ara\u015ft\u0131rma y\u00f6ntemidir. Bu y\u00f6ntemle numunelerdeki ara\u015ft\u0131r\u0131lmas\u0131 hedeflenen proteinin b\u00fcy\u00fckl\u00fc\u011f\u00fc, konsantrasyonu ve ara\u015ft\u0131rma gruplar\u0131na g\u00f6re nicelik k\u0131yaslamas\u0131 yap\u0131labilir. Western Blot tekni\u011fi yedi a\u015famada yap\u0131labilir; 1. \u00d6rneklerin haz\u0131rlanmas\u0131: H\u00fccre veya dokulardan… <\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-185","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/2021.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/185","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/2021.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/2021.brs.org.tr\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/2021.brs.org.tr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/2021.brs.org.tr\/index.php\/wp-json\/wp\/v2\/comments?post=185"}],"version-history":[{"count":5,"href":"https:\/\/2021.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/185\/revisions"}],"predecessor-version":[{"id":363,"href":"https:\/\/2021.brs.org.tr\/index.php\/wp-json\/wp\/v2\/pages\/185\/revisions\/363"}],"wp:attachment":[{"href":"https:\/\/2021.brs.org.tr\/index.php\/wp-json\/wp\/v2\/media?parent=185"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}